Starting Concentration and Percent Yield

Three starting concentrations were purified using RNAClean XP. The average for each starting concentration is shown in the graph. The three starting concentrations all had a percent yield that was similar. The average recovery from the three cleanup experiments was 78% with a σ2 of 10%.

 

1. Bind RNA to magnetic beads
2. Separate beads from contaminants
3. Wash magnetic beads with 70% ethanol to remove contaminants
4. Elute RNA from magnetic beads
5. Transfer to new plate