New! SPRIworks HT for Illumina* NGS Platforms!
High throughput Biomek -based solution prepares up to 96 libraries with size selection in just 6 hours or 3 hrs with no size selection. In addition to library construction, the system offers per well size selection so samples for multiple applications can be processed on the same plate. For added efficiency, downstream methods for quantitation, normalization and pooling are included.

Sample Prep Freedom for High Throughput Next Generation Sequencing Labs

Proven SPRI chemistry is combined with the flexibility of the Beckman Coulter Biomek FX^P liquid handler to revolutionize how next generation sequencing samples are prepared. All chemistry needed to prepare libraries is included in a kit formatted for high throughput processing. Also included are reagents to perform size selection, PCR setup and cleanup. The only thing left to do is add the DNA sample and adapter of choice.

Options, options, options…because SPRIworks HT is automated on the Biomek automated liquid handler, users have many options for how they process their samples.
 

Key Features:
•	Process up to 96 samples with size selection in less than 6hrs. 3hrs if no size selection.
•	SPRI- Based per well size selection enables processing samples for multiple applications in a single plate.
•	Quick Implementation with validated methods standardized process
•	Automated methods for all major steps. Run them all or pick and choose what fits in your day.
	»	Library Construction
	»		Size Selection
	»		PCR Setup
	»		Quantitation*
	»		Normalization
	»		Sample pooling for multiplexing
•	Reproducible and consistent library output
•	Reduced sequencing cost/sample
•	Improved sequencing service turn-around time
•	Libraries compatible with:
	»		RNA-Seq, CHiP-Seq and Target capture applications
	»		Regular fragment or paired end adapters

Increase Efficiency and Throughput

• Up to 96 high quality, fully constructed and sized libraries in under 6 hours
• Up to 25 times the throughput of manual library construction, similar to having 25 additional people to do next generation sequencing sample preparation

Beckman Coulter SPRIworks vs Manual Processing Methods

The SPRI chemistry has been optimized to purify fragmented DNA and size select the prepared library. Three different size ranges are available, as well as no size selection for processing amplicons. This feature replaces the need for gel purification and column based clean-up.

•	Short Fragments 150-350 bp (insert sizes)
•	Medium Fragments 250-450 bp (insert sizes)
•	Long Fragments 350-700 bp (insert sizes)
•	No Size Selection

Size selection range for short (A), medium(B) and long (C) fragment sizes (Agilent high sensitivity DNA chip).



Resulting PCR yields in nM, as determined by qPCR, for 1 µg of E.coli processed through the SPRIworks HT method suite. Library elution volume was 20 µL, PCR input volume was 20 µL and was cycled 10 times.

System Specifications

Up to 96 high quality, fully constructed and sized libraries in under 6 hours.

 

System Components

SPRIworks HT Fragment Library Kit 48 rxn- For Illumina NGS	B06938
SPRIworks HT Biomek FXP Dual Multi channel 96 and Span 8 (includes B13563, SPRIworks HT Methods CD)	B05450
SPRworks-HT Consumables Bundle FXP (96X10)	B05451
SPRworks-HT Methods CD (Illumina)	B13563