Beckman Coulter

    Gallios™ Flow Cytometer

    Lasers: 10 colors, 3 lasers (5 + 3 + 2 configuration) 
    Flow Product Line: Gallios™
    Flow Product Type: Instruments 
    Regulatory Status: RUO  ​



    The Gallios Flow Cytometer is a research system that delivers analytical excellence by coupling extraordinary sensitivity, resolution and dynamic range with high-speed data collection. Along with the unprecedented detection capabilities of the instrument, the Gallios includes easy-to-use software and automation to facilitate superior performance of multi-color flow cytometry assays.

    Designed to fit your needs, today and tomorrow, the Gallios flow cytometer provides efficient acquisition of excellent quality data from up to 10 colors with advanced optical design for enhanced sensitivity for multicolor assays. With customized forward scatter, to achieve the most accurate size measurement, and optimized electronics for high resolution and fast, accurate processing, the Gallios was designed for your research needs.

    Gallios is optimized for unparalleled signal to noise resolution in all parameters and its quick and easy setup of automated walk-away processing improves your workflow while its remote diagnostics minimize downtime and allow you to maximize lab productivity.

    Developed for the research and biopharmaceutical markets, with a particular emphasis on optical and electronics design, combined with powerful automation and software tools, The Gallios enhances the efficiency and analytical capabilities of flow cytometry laboratories. Electronics provide accurate and efficient digital signal processing at high event rates over a wide dynamic range of fluorescence intensities. These benefits are packaged in a compact analyzer that delivers stable performance over long periods of time and across a wide range of operating temperatures.




    The Gallios offers up to four highly reliable, solid-state lasers in standard red and blue, with violet and yellow lasers available as an option. By incorporating the optional 405nm violet laser and an optional 561nm yellow laser, the Gallios Flow Cytometer offers a greater choice of fluorochromes to perform sophisticated multicolor experiments.  With the new 561nm laser system you can analyze multiple fluorescent proteins simultaneously, as well as benefit from a greater ability to detect red fluorescent proteins. Easily interchangable optical filters facilitate detection of a variety of dyes and wavelengths.

    An innovative forward-scatter detector provides up to three measurements of cell size and visualization of particles down to 0.404 µm in diameter. A side-scatter detector incorporates an independently focused, high-performance photodiode with electronic attenuation. Six fluorescence detectors provide simultaneous acquisition of up to six fluorescence signals. Up to four fluorescence detectors can be added to the system, enabling concurrent reading of up to 10 colors. Simultaneous measurements of integral, peak and width are available for all parameters, including scatter and fluorescence signals. A selection from up to 62 parameters can be processed per analysis, at acquisition rates of 25,000 events-per-second, with high yield. Four scalable configurations allow for the future addition of more lasers and fluorescence detectors as the applications for complex multi-color flow cytometry expand.

    Available in 4 configurations
    6 color, 2 laser (488nm Blue & 638nm Red) (5 + 1)
    8 color, 2 laser (488nm Blue & 638nm Red) (5 + 3)
    10 color, 3 laser (488nm Blue, 638nm Red & 405nm Violet) (5 + 3 + 2)
    10 color, 4 laser (488nm Blue & 561nm Yellow [co-linear], 638nm Red, 405nm Violet) (5 + 3 + 2)
    Innovative laser cartridges
    Picomotor driven beam steering optics
    Laser control to conserve laser life
    Design for laser flexibility
    Customer temperature regulation Plug and play filters allow rapid instrument reconfiguration between experiments
    Filters are easily accessible and interchangeable with no tools required

    Software features:
    Multi-User Administration
    Drag & Drop replay of data
    Boolean gating and color eventing hierarchy
    New log/lin transform for visualization of negative data and compensation optimization
    Automatic PDF and .xls creation Instrument Dashboard
    LEDs clearly indicate instrument status from across the lab
     Efficient delivery of light
    More power at the flow cell for greater resolution in your applications
    Efficient and reliable solid state blue, red, and violet lasers
    Free space delivery of laser light
    Optimized beam steering optics
    Increased power at flow cell

    Scatter Parameters
    Efficient delivery of light
    3 unique setting for relative size measurements
    Innovative submicron particle resolution setting for forward scatter
    Independent side scatter alignment

    Electronics design incorporates fast, accurate measurement of signals over a wide range of event rates
    Fast pulse recovery
    Minimize linearity errors
    Sensitivity of dim particles
    Minimize dead time
    Accuracy at high event rates

    PRO Service Compatible

    Gallios - Optics in Action
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     Optics in Action
    The Gallios offers up to four solid state diode lasers.  The lasers are independently focused in the auto-beam tower using an innovative steering design mechanism that provides optimal excitation at the flow cell for reliable high quality results.

    The emitted energy is delivered using fiber optics to the boulevard.  The patented boulevard design uses 18 degree reflective optics which results in greater light collection efficiency to better resolve data, especially dim markers.  The boulevard has been optimized to provide application flexibility with easy access plug and play filters and for the collection of tandem dyes exclusively available from Beckman Coulter.



    Gallios - Electronics Explained
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     Electronics Explained

    Optimized for high resolution and fast, accurate processing
    Sensitivity and resolution are accomplished with excellent digital electronics design.
    The Gallios maintains a high yield of processed signals, greater than 90% even at 25k events per second.

    • 20 bit resolution
    • 1,048,576 channel display
    • More channels of resolution to separate even the dimmest of populations

    Excellent accuracy and resolution, for dim stains and for quantitation
    Patented electronics design incorporates fast, accurate measurement of signals over a wide range of event rates. 

    • Higher bits of information
    • Baseline restoration 
    • Fast signal processing
    • Minimize data loss

    High yield electronics with fast pulse recovery at high speeds
    Gallios’ electronic system minimizes linearity errors and adjusts for temperature variability.

    • Fast pulse recovery
    • Sensitivity of dim particles
    • Minimize dead time
    • Accuracy at high event rates

    Unprecedented analytical excellence across the operating range
    Changes to room temperature can affect measurement by more than 25% affecting mean channel and CV, and ultimately compensation and reproducibility especially with longitudinal assays. Gallios electronics have a self-correcting temperature regulation system. 

    • Adjusts for temperature variability
    • Minimizes linearity errors based on temperature variations