Molecular Devices
- SpectraMax M5/M5e
다섯 가지 이상의 광학 측정이 가능한, 최고의 성능과 높은 감도를 자랑하는
Advanced Multi-Detection Microplate Reader
Monochromator System을 이용하여 ABS, FI, LUM, TRF, FP, TR-FRET 및 htrf 실험을 수행할 수
있으며, Cuvette Port를 장착하여 ABS, FI, LUM Spectrometer 기능까지 겸비한 다재다능한
Multi-Detection Microplate Reader 입니다.
⊙ ABS / Fl / LUM / TRF / FP
ABS (200 - 1000 nm)
FI (250 - 850 nm)
LUM (250 - 850 nm)
TRF (250 - 850 nm)
FP (300 - 750 nm)
htrf (M5e Only)
⊙ Xenon Flash Lamp
⊙ Monochromator
⊙ 6 ~384 -Well Formats
⊙ Top / Bottom Read
⊙ Cuvette (ABS, FI, LUM)
⊙ Shaking
⊙ Temp. Control [~45'C]
⊙ Endpoint / Kinetic / Spectrum Scan/ Well Scan
General Specifications | ||
Dimensions (in.) | 8.6 H x 22.8 W x 15.3 D | |
Dimensions (cm) | 22 H x 58 W 39 D | |
Weight | 35-36 lbs (15.9-16.4kg) | |
Power Consumption | < 125 Watts | |
Power Source | 100-240 VAC, 3 A, 50/60 Hz | |
Robotic Compatible | Yes | |
Generic Photometric Performance | ||
Plate Formats | 6, 12, 24, 38, 96, 384 wells | |
Light Source | Xenon Flash Lamp (1 joule/flash) | |
Detectors | 1-2 photomultiplier tubes | |
ShakerTime | 0 to 999 seconds | |
Temperature Control | 2-4 °C above ambient to 45-60 °C | |
Temperature Uniformity | < 1°C at 37°C set point | |
Temperature Accuracy | ±1°C at 37°C set point | |
Endpoint Reading | All modes | |
Kinetic Reading | All modes | |
Spectral Scanning | All modes | |
Well Scanning | Abs, FI, TRF, Lum | |
Standard Read Times (minutes:seconds)†† | ||
| 96 wells | 384 wells |
Absorbance | 0:18 | 0:49 |
Fluorescence Intensity | 0:17 | 0:48 |
Fluorescence Polarization | 0:42 | 2:03 |
Time-Resolved Fluorescence | 0:17 | 0:48 |
Luminescence | 2:00 | 7:00 |
Absorbance Photometric Performance | ||
Reading Capabilities | Cuvette or microplate | |
Wavelength Range | 200-1000nm | |
Wavelength Selection | Monochromator, tunable in 1.0 nm increments | |
Wavelength Bandwidth | < 4.0nm | |
Wavelength Accuracy | ±2.0 nm | |
Wavelength Repeatability | ±2.0 nm | |
Photometric Range | 0-4.0 OD | |
Photometric Resolution | 0.001 OD | |
Photometric Accuracy (Microplate) | < ±0.006 OD ±1.0%, 0-2 OD | |
Photometric Accuracy (Cuvette) | < ±0.005 OD ±1.0%, 0-2 OD | |
Photometric Precision | < ±0.003 OD ±1.0%, 0-2 OD | |
Stray Light | < 0.05% @ 230 nm | |
Fluorescence Intensity Performance | ||
Reading Capabilities | Cuvette or top or bottom of a microplate | |
Wavelength Range | 250-850 nm | |
Wavelength Selection | Monochromators, tunable to 1.0 nm increments | |
Bandwidth (Ex, Em) | 9nm, 15 nm | |
Optimized sensitivity | ≤ 1 pM fluorescein in 96 wells | |
Guaranteed sensitivity** | < 5 pM fluorescein in 96 wells or cuvette | |
Luminescence Performance | ||
Reading Capabilities | Cuvette or top or bottom of a microplate | |
Wavelength Selection | All wavelengths or with selected wavelengths | |
Wavelength Range | 250-850 nm | |
Optimized sensitivity | ≤ 43 pM ATP in 96 wells | |
Guaranteed sensitivity** | ≤ 75 pM ATP in 96 wells | |
Cross-talk | < 0.3% in 96- and 384-well microplates | |
Time Resolved Fluorescence Performance | ||
Reading Capabilities | Top or bottom of a microplate | |
Wavelength Range | 250-850nm | |
Wavelength Selection | Monochromators, tunable in 1.0 nm increments | |
Bandwidth (Ex, Em) | 9nm, 15 nm | |
Precision Data Collection | 1-100 flashes, delay of 0-600 µsec before read, integration time selectable between 50-1500 µsec | |
Optimized sensitivity** | ≤ 10 fM europium in 96 wells | |
Guaranteed sensitivity** | ≤ 100 fM europium in 96 or 384 wells | |
SpectraMax M5e reader only | Certified to Cisbio Bioassays' HTRF assays performance Specifications | |
Fluorescence Polarization Performance | ||
Wavelength Range | 300-750 nm | |
Wavelength Selection | Monochromators , tunable in 1.0 nm increments | |
Bandwidth (Ex, Em) | 9 nm, 15 nm | |
Guaranteed precision** | ≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells | |
