General Specifications

Dimensions (in.)

8.6 (H) x 22.8 (W) x 15.3 (D)

Dimensions (cm)

22 (H) x 58 (W) x 39 (D)

Weight

36 lbs (16.4 kg)

Power Consumption

< 420 watts

Power Source

100-240 VAC, 3 A, 50/60 Hz

Robotic Compatible

Yes

General Photometric Performance

Plate Formats

6, 12, 24, 48, 96, 384 wells

Light Source

Xenon Flash Lamp (1 joule/flash)

Detectors

2 photomultiplier tubes

ShakerTime

0 to 999 seconds

Temperature Control

2 °C above ambient to 60 °C

Temperature Uniformity

< 1°C at 37°C set point

Temperature Accuracy

±1°C at 37°C set point

Endpoint Reading

All modes

Kinetic Reading

All modes

Spectral Scanning

All modes

Well Scanning

Abs, FI, TRF, Lum

Standard Read Times (minutes:seconds)^††

96 wells

384 wells

Absorbance

0:18

0:49

Fluorescence Intensity

0:17

0:48

Fluorescence Polarization

0:42

2:03

Time-Resolved Fluorescence

0:17

0:48

Luminescence

2:00

7:00

Absorbance Photometric Performance

Reading Capabilities

Cuvette or microplate

Wavelength Range

200-1000 nm

Wavelength Selection

Monochromator, tunable in 1.0 nm increments

Wavelength Bandwidth

< 4.0 nm

Wavelength Accuracy

±2.0 nm

Wavelength Repeatability

±0.2 nm

Photometric Range

0-4.0 OD

Photometric Resolution

0.001 OD

Photometric Accuracy (Microplate)

< ±0.006 OD ±1.0%, 0-2 OD

Photometric Accuracy (Cuvette)

< ±0.005 OD ±1.0%, 0-2 OD

Photometric Precision

< ±0.003 OD ±1.0%, 0-2 OD

Stray Light

< 0.05% @ 230 nm

Fluorescence Intensity Performance

Reading Capabilities

Cuvette or top or bottom of a microplate

Wavelength Range

250-850 nm

Wavelength Selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (Ex, Em)

9 nm, 15 nm

Optimized sensitivity

≤ 1 pM fluorescein in 96 wells
≤ 1.5 pM in 384 wells

Guaranteed sensitivity**

< 5 pM fluorescein in 96 wells or cuvette
≤ 20 pM in 384 wells

Luminescence Performance

Reading Capabilities

Cuvette or top or bottom of a microplate

Wavelength Selection

All wavelengths or with selected wavelengths

Wavelength Range

250-850 nm

Optimized sensitivity

≤43 pM ATP in 96 wells

Guaranteed sensitivity**

≤75 pM ATP in 96 wells

Cross-talk

< 0.3% in 96- and 384-well microplate

Time-Resolved Fluorescence Performance

Reading Capabilities

Top or bottom of a microplate

Wavelength Range

250-850 nm

Wavelength Selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (Ex, Em)

9 nm, 15 nm

Precision Data Collectionn

1-100 flashes, delay of 0-600 µsec before read, integration time selectable between 50-1500 µsec

Optimized sensitivity

≤10 fM europium in 96 wells

Guaranteed sensitivity**

≤100 fM europium in 96 or 384 wells

SpectraMax M5e reader only

Certified to Cisbio Bioassays' HTRF assays performance Specifications